reverse transcriptase

Expression of Telomerase RNA and Telomerase Reverse Transcriptase mRNA in Lesions of Psoriasis Vulgaris

寻常性银屑病皮损端粒酶RNA、反转录酶mRNA表达

The replication of HBV DNA passes through reverse transcription. As the reverse transcriptase is lack of effective function of proofreading the bases, the HBV genome is easier to mutate than other DNA viruses.

HBV复制过程中要经过逆转录，由于逆转录酶缺乏有效的碱基校对功能，故HBV基因组比一般DNA病毒易发生变异。

Differential Inhibitory Effects and Separation of Melastoma normale Extracts on the Activities of HIV Reverse Transcriptase and DNA Polymerase

羊开口提取物的分离及对艾滋病毒逆转录酶和DNA多聚酶活性的抑制作用

Third, for any virus that did enter cells, the microbicide would block an enzyme, reverse transcriptase, that the virus needs to replicate.

第三，对于任何进入细胞的病毒来说，抗生素可以阻断一种病毒赖以复制的逆转录酶。

Method RhD mRNA was analyzed through reverse transcriptase PCR ( RT-PCR) and cDNA sequencing in 4 individuals with different Rh phenotypes ( CcDDEe 、 CCDDee 、 CcDDee and ccDdee).

方法采用逆转录PCR（RT PCR）技术检测4名不同Rh表型（CcDDEe、CCDDee、CcDDee和ccDdee）个体的RhD mRNA，然后进行cDNA测序分析。

Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

Roles of the EnvZ/ OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR ( qRT-PCR).

同时通过突变分析、定量逆转录聚合酶链反应（qRT-PCR）研究了EnvZ/OmpR二元系统在应对渗透压力中的作用。

Moreover, the detection limit had been improved by the use of Superscript reverse transcriptase.

并且，使用了Superscript反转录酶进行反转录，提高了检测的灵敏度。

Expression of telomerase reverse transcriptase and telomeric repeat binding factor 2 during rat myocardial development

端粒酶逆转录酶和端粒重复结合因子2在大鼠心肌细胞发育过程中的表达

The Study of the Immortalization of Human Bone Marrow Mesenchymal Stem Cells by Transducting Human Telomerase Reverse Transcriptase Gene

转导人端粒酶逆转录酶基因致人骨髓间充质干细胞永生化的研究

The expression of TLR 4, MD-2 mRNA were assayed by reverse transcriptase polymerase chain reaction ( RT-PCR ).

逆转录聚合酶链反应 ( RT -PCR ) 检测各组细胞TLR4和MD-2mRNA的表达.

Objective To establish polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT-PCR) for detecting human cytomegalovirus ( HCMV) and to find out the relationship between diabetes mellitus ( DM) and HCMV infection.

目的建立检测人巨细胞病毒（HCMV）感染的聚合酶链反应/逆转录&聚合酶链反应（PCR/RT-PCR）方法，并探讨糖尿病与HCMV感染的关系。

Objective: To study the human telomerase reverse transcriptase ( hTERT) activity and expression of PTEN protein in squama cell carcinoma of tongue and to investigate their relations during histogenesis and development of squama cell carcinoma of tongue.

目的：探讨舌鳞状细胞癌组织中hTERT与PTEN蛋白表达的相关关系，为治疗原发性舌鳞癌提供理论依据。

Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reverse transcriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。

Restriction enzymes and reverse transcriptase are keys to gene engineering.

限制性酶和逆转录酶成为基因工程的重要手段.

Methods R and/ or G banding technique was used for analysis of karyotype. DNA blotting for HRX gene rearrangement, and polymerase chain reaction ( PCR) and reverse transcriptase PCR ( RT PCR) for fusion gene detection.

方法用R带和（或）G带分带技术进行核型分析，DNA印迹法检测HRX重排，聚合酶链反应和逆转录聚合酶链反应进行融合基因检测。

Telomerase is a specialized reverse transcriptase single-stranded chromosome ends and oligonucleotides in vivo and in vitro.

端粒酶是一种特殊的逆转录酶,它能在体内延伸染色体 单链 末端,在体外延伸寡聚核苷酸.

Detection for HCV with FD thermostable reverse transcriptase mediated RT nested PCR

FD耐热逆转录酶介导的逆转录nested PCR检测丙型肝炎病毒

Results: Telomerase is a reverse transcriptase comprised of many subunit.

结果端粒酶是一多亚基复合物,具有逆转录酶活性.

Method The rotavirus genome in the plasma samples of 60 infants with rotavirus diarrhea and in the peripherial blood mononuclear cells ( PBMCs) of 14 out of the infants who showed abnormal serum enzyme levels were subjected to nest reverse transcriptase polymerase chain reaction test.

方法应用套式一逆转录PCR方法检测60例RV腹泻患儿外周血血浆和其中14例血清酶学异常患儿外用血单个核细胞中的RV。

Expression of Human Telomerase Reverse Transcriptase and in Genitalia Leukoplakia and Squamous Cell Carcinoma of Vulva

人端粒酶逆转录酶在外阴白斑和外阴鳞癌中的表达

Research Progress in Targeting Human Telomerase Reverse Transcriptase ( hTERT) Promoter in Cancer Gene Therapy

靶向人端粒酶逆转录酶启动子在肿瘤基因治疗中的研究进展

The reverse transcriptase telomerase is able to maintain stable telomeric length and essential for the unlimited proliferation of tumor cells. Telomerase activity has been detected in 85-90% of human tumors and tumor-derived cell lines but not in most human somatic cells.

端粒酶表达于85%以上的肿瘤细胞中，能够维持细胞中端粒的长度，对永生化细胞的增殖具有重要作用，而在大多数人正常体细胞中却没有表达。

There are thousands of reverse-transcriptase genes in the genomes of all mammals.

在所有哺乳动物的基因组中有上千个反转录酶的基因.

In the present study, reverse transcriptase polymerase chain reaction ( RT-PCR) was used to detect the changes of the mRNAs expression of 5-HT_ 2~ 7 receptor subtypes in the rat dorsal root ganglia ( DRG) induced by spared nerve injury ( SNI).

本研究利用反转录聚合酶链式反应（RT-PCR）方法，观察了坐骨神经分支选择性损伤（SNI）所致神经病理性痛条件下，大鼠背根神经节（DRG）中5-HT2~7受体亚型mRNAs的时程表达变化。

After sacrificing the rats in twelve weeks, serum and aorta were studied through the techniques: biochemistry, radioimmunoassay, immuno-histochemistry ( IHC), reverse transcriptase PCR ( RT-PCR) and western blot.

各组大鼠实验12周后处死，提取血清、主动脉、通过生化、放免、免疫组化、RT&PCR法及蛋白印迹杂交法进行研究。

Methods Gene expression associated with vascular channel in osteosarcoma endothelial cell was measured by semi-quantitative reverse transcriptase polymerase chain reaction using the transcriptional level of the house-keeping gene for internal calibration.

方法利用RT-PCR技术对骨肉瘤肿瘤细胞（MG63、U2OS）血管形成相关基因进行检测，并同内皮细胞（ECV304）相比较。

Telomerase is a reverse transcriptase that adds telomeric repetitive sequence to chromosomal ends.

端粒酶是一种将端粒区的重复序列加到染色体末端的逆转录酶。

C fos gene expression level was semi quantified by reverse transcriptase ( RT) PCR.

采用逆转录（RT）PCR对C Fos基因表达水平进行半定量分析。

Immortalization of cultures derived from primary breast carcinoma by ectopic expression of human telomerase reverse transcriptase and its significance

端粒酶永生化原始乳腺癌组织来源细胞及其意义